APICHELA Silvana Andrea
Detection of the Matrix Metalloproteinases MMP-2 and MMP-9 and Tissue Inhibitors of Metalloproteinases TIMP-1 and TIMP-2 in Llama (Lama glama) oviduct
ZAMPINI, RENATO; ARGAÑARAZ, MARTÍN E; MICELI DORA C.; APICHELA, SILVANA A.
REPRODUCTION IN DOMESTIC ANIMALS (1990)
WILEY-BLACKWELL PUBLISHING, INC
Lugar: Londres; Año: 2014 vol. 3 p. 492 - 498
Martix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in several reproductive events like oocyte-spermatozoa interaction and semen liquefaction. In order to study their role in the llama oviductal reproductive process MMP activity in oviductal fluid (OF) was assayed. Considering that llama genome sequences are partially known, a strategy to procure cDNA sequences of MMP-2, MMP-9, TIMP-1 and TIMP-2 was designed. Afterwards, their expression patterns in the different llama oviductal segments were assayedGelatine zymograms detected 62 and 94 kDa protease activities that matched MMP-2 and proMMP-9, respectively. Expression pattern analysis showed that MMP and TIMP mRNAs were present in ampulla, isthmus, utero-tubal junction and papilla. Altogether, these findings support the argument that MMPs/TIMPs are produced in the oviduct and secreted into the oviductal lumen. Our results encourage further studies to elucidate the role of these proteins in reproductive oviductal events, which would contribute to the development of assisted reproductive technologies in South American Camelids.