CURRENT ADENOVIRUS GENOTYPES, INCLUDING GENOTYPE 66/7h, IN CHILDREN WITH ACUTE RESPIRATORY INFECTION: A FIFTEEN-YEARS STUDY PERIOD

MARCONE DN; LABADIE BRACHO M; VIALE D; TRENTACOSTE A; ELLIS ALEJANDRO; CARBALLAL GUADALUPE; ECHAVARRIA M; REYES NOELIA; RICARTE C; VIDAURRETA S

Savannah

Simposio; 35nd ASM Clinical Virology Symposium and Annual Meeting PanAmerican Society for Clinical Virology; 2019

Human Adenoviruses (AdV) are divided into species (A-G) and were traditionally classified intoserotypes by neutralization and into genome types by restriction enzyme analysis. Currently,AdV are classified into genotypes by sequencing analysis and phylogenomics. Respiratoryinfections are principally associated to species B, C and E. In particular, AdV genome type 7 ?h?renamed as AdV 66 by phylogenomics, has been associated with higher severity and evendeath in children with respiratory disease.The goal of this study was to determine whether AdV 66/7h, remains circulating in childrenwith acute respiratory infection (ARI) and to describe circulating genotypes in the last andcurrent years in Buenos Aires, Argentina.Mat y MetHospitalized children with ARI from two hospitals were evaluated. From 2000-2005, childrenfrom Garrahan Hospital (a terciary children hospital) were studied and from 2008-2018,patients from CEMIC (a general hospital) were evaluated. Clinical and epidemiologicalcharacteristcs of AdV positive patients were analyzed. AdV typing was performed by partialhexon sequencing followed by phylogenetic analysis with reference strains by Maximumlikelihood (PhyML).ResultsDuring the 15 years study period, 141 HAdV positive children with respiratory infection wereselected. Of them, 134 (96.5%) could be typed. Phylogenetic analysis reveals the presence of 4HAdV species and 11 circulating genotypes. HAdV species B (52.9%) and C (37.3) were themost frequent, followed by species E (8.2%) and D (1.5%). Genotype HAdV-B3 was thepredominant ( 28.4%), followed by HAdV-C2 (17.9%), HAdV-B66 (14.9%), HAdV-C1 (13.4%),HAdV-E4 (8.2%), HAdV-B55 (8.5%), HAdV-C5 (6.0%), HAdV-D8 (1.5%), HAdV-B11 (0.8%), HAdV-B35 (0.8%) and HAdV-B68 (0.8%).AdV 66/7h was detected only from 2000 to 2005. This strain was the most frequently detectedfrom 2000 to 2003 (43%) and from 2004 its frequency started to decrease. Genotype 3 startedto be the most frequent circulating genotype since 2004. Of 78 AdV positive children fromGarrahan hospital, 6 died and genotypes 1, 3 and 66/7h were detected from these fatal cases.ConclusionsAdV 66/7h was the predominant genotype from 2000-2005 but was no longer detected in thelast years. AdV 66/7h was detected in fatal cases as well as genotypes 1 and 3. A variety ofdifferent genotypes were detected in hospitalized children with ARI during this 15-yearsperiod.