Evaluation of the surface properties of Lactobacillus isolated from bullfrog hatcheries

PASTERIS S.E.; VERA PINGITORE E.; ROIG BABOT G.; BÜHLER M.I; NADER-MACIAS M.E.

Tafi del Valle - Tucumán- Argentina

Congreso; XXIII JORNADAS DE LA ASOCIACION DE BIOLOGIA DE TUCUMAN; 2006

Asociación de Biología de Tucumán

Evaluation of the surface properties of Lactobacillus isolated from bullfrog hatcheries S.E. Pasteris1; E. Vera Pingitore1; G. Roig Babot1; M.I. Bühler1; M.E. Nader-Macías2 1INSIBIO-CONICET - Facultad de Bioquímica, Química y Farmacia-UNT y 2CERELA-CONICET. Chacabuco 461. C.P.4000. Tucumán. pasteris@fbqf.unt.edu.ar The bullfrog-hatcheries have been a growing activity all around the world during the last decade. However, some diseases such as red-leg syndrome have a negative effect on the production expenses. Our studies are focused to the use of probiotics to prevent bacterial diseases in hatcheries. Thus, the inhibitory activity of indigenous lactic acid bacteria from different areas of a hatchery was evaluated. In this work, we analysed the surface properties of  lactobacilli as a predictive measure of its adhesion to epithelial cells by studying both, hydrophobicity and haemagglutination degrees. Hydrophobicity was determined by the hexadecane partition method while haemagglutination ability was tested by using human red cells. The hydrophobicity assays showed that the strains had a moderate to low degree according with the hydrophilic nature of the mucus skin. Only seven strains were able to haemaglutinate. The highest values were shown by  Lactobacillus GST27, FZ6´ and AM11 isolated from the skin of healthy animals, EP35A from freshwater of healthy animals and 9T from balanced feed. The relationship between surface properties and inhibitory activity allowed us to select Lactobacillus AM11, FZ6’, FZ17 and 9T as potential microorganisms to be used as probiotics for R. catesbeiana hatcheries.