INVESTIGADORES
DUPUY Fernando Gabriel
congresos y reuniones científicas
Título:
Effect of growth temperature on the properties of Listeria membrane and on the interaction with the antimicrobial peptide enterocin CRL35.
Autor/es:
MEDINA AMADO, CAROLINA; DUPUY, FERNANDO G.
Lugar:
San Luis
Reunión:
Congreso; XVIII Reunión Anual de la Sociedad Argentina de Biofísica; 2019
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
The food borne pathogen Listeria monocytogenes is able to grow in a wide variety of environmental conditions, such as temperature. In this regard, the bacteria endure 4 °C and up to 37 °C as well. Membrane adaptation is an important biochemical process for maintaining steady bilayer elasticity, necessary for proper membrane protein functionality and membrane selective permeability. In this work, we studied the lipidic extract of Listeria cultures growth at 10, 25 and 37 °C by means of bilayer and monolayer model systems. The extracts obtained from cultures at 10 °C were more disordered than 25 and 37 °C, according to infrared spectroscopy and fluorescence anisotropy. Also, Langmuir monolayers assays indicated that lipids obtained from 10 °C cultures formed softer films than lipids from 25 and 37 °C. Results thus indicate that the cells adjust membrane properties according to homeoviscous adaptation theory by means of lipid composition. Changes in membrane properties affected enterocin CRL35 antimicrobial activity, which was probed in Listeria cells at different temperatures.