INVESTIGADORES
DUPUY Fernando Gabriel
congresos y reuniones científicas
Título:
Structural and functional studies of bacterial model membranes and peptide interaction
Autor/es:
MEDINA AMADO, CAROLINA; GARNICA, MAURO; DE MARCO, NILDA; OLIVEIRA, RAFAEL; DUPUY, FERNANDO G.
Lugar:
La Plata
Reunión:
Simposio; XLVII Reunión Anual de la Sociedad Argentina de Biofísica; 2018
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
Novelantimicrobial therapies are needed as a result of the increasing number ofmicrobials resistant to ordinary antibiotics. In this regard, bacterialmembranes represent an important molecular target for new antimicrobials likeamphipathic peptides. The bacterial lipid bilayer is typically composed by ahigher amount of anionic lipid, compared to eukaryotic membranes. Also,nonbilayer structures can be formed by some of the main bacterial lipids, likephosphatidylethanolamine (PE) or even phosphatidylglycerol (PG) and cardiolipin(CL), when negative charge is screened. In this work, bacterial model membraneswere studied by means of x ray diffraction techniques in order to assessstructural rearrangements produced by interaction with antimicrobial peptides.In addition, functional and molecular properties as determined by infrared,fluorescence spectroscopy and Langmuir monolayers were investigated. Theresults indicate that bilayer composition can modulate themechanisms by which peptides interact and perturb membrane properties indifferent ways. Thus, there is none a single mechanism of action of peptideantimicrobial activity, but it rather depends on the intrinsic properties ofeach particular membrane.
