INVESTIGADORES
DUPUY Fernando Gabriel
congresos y reuniones científicas
Título:
Thermotropic and structural study of sphingolipid mixtures
Autor/es:
DUPUY, FERNANDO GABRIEL; OLIVEIRA, RAFAEL; MAGGIO, BRUNO
Lugar:
Carlos Paz, Córdoba
Reunión:
Congreso; XLII Reunión Anual de la Sociedad Argentina de Biofísica; 2013
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
The hydrocarbon chain of the lipids is a major determinant of the phase behavior of these molecules: long and saturated acyl chains induce gel/solid ordered phases with a high degree of intermolecular association. However, lipid phase states can be dramatically altered in multicomponent systems by non-ideal mixing. In Langmuir monolayers, we demonstrated the formation of an azeotropic mixture between the short chain ceramide C10:0 Cer and the sphingomyelin N-acylated with palmitic acid C16:0 SM, in which a condensed phase is obtained at surface pressure values where both components are in expanded state. In this work, we studied the thermotropism of the sphingolipids C10:0 Cer, C16:0 SM and their binary mixtures by high sensitivity differential scanning calorimetry. Also, structural information of the different lipid dispersions was obtained from small angle synchrotron X-ray diffraction. The results indicate lipid mixing in the gel state but phase separation upon melting. Also, bilayer mesomorphism was stabilized in the mixed state, although pure C10:0 Cer formed an unusually ordered inverted phase.