INVESTIGADORES
DUPUY Fernando Gabriel
congresos y reuniones científicas
Título:
Mixed monolayers of sphingomyelins and ceramides with different N-acyl chain lengths show favorable interactions and stable condensed phases
Autor/es:
DUPUY, FERNANDO; MAGGIO, BRUNO
Lugar:
San Javier, Tucumán
Reunión:
Congreso; XLI Reunión Anual de la Sociedad Argentina de Biofísica; 2012
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
Sphingolipids are structural components of biomembranes involved in cell signaling, owing to the interaction with specific lipids, the ability of modify the bilayer order degree and induce segregation of or even change of phase state upon enzymatic modification. However, a detailed characterization of the behavior of pure molecular species and of their mixtures has not yet been done. In this work, sphingomyelins and ceramides with different lengths and unsaturation degree of the N-acyl chain were studied by means of Langmuir monolayers in order to assess the mean molecular area, the resultant perpendicular dipole moment and the lateral compressibility of the pure species and their binary mixtures as a function of the lateral pressure and composition. The results indicate that in mixtures of sphingomylelins and ceramides, favorable molecular interactions exist leading to the formation of monolayers with decreased phase-transition pressures and lateral compressibility. The study of the lateral topography of the films by Brewster angle microscopy confirmed that the sphyngomyelin-ceramide mixtures showed more condensed states than the pure components when compared at the same surface pressures