IPATEC   26054
INSTITUTO ANDINO PATAGONICO DE TECNOLOGIAS BIOLOGICAS Y GEOAMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
RNA-Seq Based Transcriptome Analysis Reveals UV-Regulated Gene Expression in a Native Isolate of Aureobasidium pullulans from Patagonia
Autor/es:
ALVAREZ L. P.; LIBKIND D.; PARRA M. ; FRANCO L.; YAÑEZ-OLIVARES C.; BELLORA N.
Lugar:
Bariloche
Reunión:
Simposio; 34º International Specialized Symposium on Yeasts; 2018
Institución organizadora:
IPATEC
Resumen:
Ultraviolet radiation (UVR) is an important harmful factor for biosphere, inducingseveral stress responses in organisms. The exposure to UVR-B (280 315 nm) can causedirect and indirect cellular damage. For organisms living in extreme conditions, dailyexposure to UVR-B ( UV from now on) acts as an environmental pressure, selectingstrategies to improve their performances against UV-detrimental effects, such as theproduction of ROS. It is known that several species of fungi are able to produce mycosporines, a biotechnologically important molecule with antioxidant and UVsunscreen activities. We predicted a gene cluster potentially responsible formycosporine biosynthesis in the genome of Aureobasidium pullulans. This cluster issimilar to that reported in the genome of the mycosporinogenic yeast Phaffiarhodozyma. The aim of this work was to characterize global changes in gene expressioninduced by UV exposure in a native isolate of A. pullulans from Patagonia through aRNA-seq-based transcriptomic approach. We compared RNA-seq results for culturesgrown at 20 °C in two conditions: constant darkness and with a 5-min UV pulse.Differential expression analysis of the resulting transcriptomic data showed that 2% ofthe genes changed their expression as a result of UV exposure, of which about 70%represented upregulated genes. Even though a 5 min UV pulse was not sufficient totrigger a massive de-regulation, our analysis support that UV participates inphotostimulated processes. As expected, among upregulated genes, we found thosebelonging to the mycosporine cluster. Surprisingly, we were also able to detect an UV-mediated induction of circadian genes. This finding triggers new questions about theeffect of UV in endogenous rhythms in fungi.