IPATEC   26054
INSTITUTO ANDINO PATAGONICO DE TECNOLOGIAS BIOLOGICAS Y GEOAMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Sharing sunscreen. A brief story of mycosporine production in Fungi
Autor/es:
DIEGO LIBKIND; NICOLAS BELLORA; LUCIA GUZMAN; MARTÍN MOLINÉ
Lugar:
Heidelberg
Reunión:
Conferencia; EMBO Conference: Experimental Approaches to Evolution and Ecology Using Yeast; 2016
Institución organizadora:
European Molecular Biology Laboratory (EMBL)
Resumen:
Several species of fungi are able to produce mycosporines, a compound that serve as a defense mechanism against UV radiation and reactive oxygen species. However, when we study the phylogenetic distribution of this character among fungi, we observe a patchy distribution which suggests that mycosporine production is a plesiomorphic character. Here we use a set of 138 genomes (from public databases) to reconstruct the phylogenetic history of mycosporine production. Genomes were annotated de novo with GeneMark, genes DDGS, OMET and ATP-G (genes of the synthesis pathway) were identified by reciprocal blast, gene phylogenies were constructed for each gene, and a phylogenomic tree was constructed using a set of 192 orthologous proteins using RaxML. Finally, we identified and characterized mycosporine production by HPLC in 48 species representing the mayor groups in fungi. Our work allows to confirm that the ability to produce mycosporines is a plesiomorphic character in Dikarya (Ascomycota+Basidiomycota), and we provide evidence supporting that all the subphyla have species that lost this ability through evolution. Moreover, we shown that the presence of the three genes DDGS, OMET and ATP-G grouped in a cluster was the original disposition and that this disposition only remains in Pezizomycotina and Agaricomycotina. Besides we notice that, contrary to all models, only two genes DDGS and ATP-G genes are essential for mycosporines biosynthesis, while the gene OMET is absent and seems to be unnecessary in Puccioniomycotina and Ustilagomycotina. Finally, it was possible to determine that various events of horizontal transfers occurs among different groups of species in the class Agaricomycetes and in the subphylum Pucciniomycotina. But more shocking we provide evidence that species of the subphylum Taphrinomycotina lost the original fungal genes, and recover the capability to produce mycosporine with genes from an unknown donor related with the clade holozoa.