Aqueous extract of the argentinean native plant Prosopis caldenia (Caldén) induces cytotoxic effects against Triple-Negative Breast Cancer cells.

MARÍA JULIA FERRONATO; ANA PAULA PEDERSOLI; ELIANA NOELIA ALONSO; AGUSTINA IBARRA; AGUSTINA GUTIERREZ; PABLO MARINANGELI; VALENTINA CLEMENTE; GEORGINA PAMELA COLÓ; MARÍA EUGENIA FERMENTO; ALEJANDRO CURINO; MARÍA MARTA FACCHINETTI

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Plant-originated drugs/formulations are employed as a complementary therapy for treating various human ailments including cancer. Recently, native species from Argentina belonging to genus Prosopis have begun to be studied for their beneficial biomedical properties. Prosopis caldenia (Pc) is locally known as ?Caldén? and it has not been studied from a medicinal point of view. The aim of this work was to investigate the antitumor potential of Pc leaves aqueous extract (PcAE) against MDA-MB-231 and 4T1 Triple Negative Breast Cancer (TNBC) cells. To this end, cell viability was studied by colorimetric crystal violet and MTT assays in cells treated with vehicle or PcAE (10-100-1000-2500-5000 ug/ml). Cell cycle was analysed by flow cytometry in cells stained with propidium iodide. Cell migration was assessed by "wound closure" assays. The results show that PcAE reduced cell viability in a concentration-dependent manner in both cell lines at 48 and 72 hours (h) of treatment (p