Fungal Endophytes in Sweet Orange Citrus sinensis (L.) Osbeck in Jujuy- Argentina.

NOEMÍ DEL V. BEJARANO, ; LEONOR CARRILLO

Asian Journal of Agricultural and Food Sciences

Asian Online Journals

Año: 2016 vol. 4 p. 54 - 59

According to the interaction established with the plant tissues, the endophytes can be mutualistic or pathogenic fungi. The objectives were to identify the fungal endophytes of the sweet orange leaves [Citrus sinensis (L.) Osbeck] from Jujuy-Argentina, and study the in vitro interactions between the isolates. The asymptomatic leaves were taken from the oldest shoots. Leaf pieces were aseptically plated on agar carrot. Isolation, culture and morphometric measurements were made. For some species, identification was performed by PCR-ITS. Inoculations on leaves and fruit orange were carried out, and antagonism test were conducted between non-pathogenic endophytes and the pathogen G. citricarpa.The fungal endophytes isolated from citrus leaves were: Colletotrichum gloeosporioides, Glomerella cingulata, Lasiodiplodia theobromae, Phomopsis citri, Guignardia citricarpa, Phyllosticta capitalensis, Cladosporium herbarum, Xylaria hypoxylon, X. multiplex, Xylaria sp., Fusarium sp., Penicillium sp., Alternaria sp., Diplococium sp.,and Stenella sp. Xylaria spp., C. gloeosporioides and P. capitalensis were found antagonists against G. citricarpa.