INVESTIGADORES
LOPEZ Fabian Enrique
congresos y reuniones científicas
Título:
IDENTIFICATION OF NOVEL RcsB-REGULON GENES IN S. Typhimurium
Autor/es:
FARIZANO J. V.; PESCARETTI M. M.; LOPEZ FABIAN E; MORERO, R.D.; DELGADO, MONICA A.
Lugar:
San Miguel de Tucuman
Reunión:
Congreso; VII CONGRESO ARGENTINO DE MICROBIOLOGÍA GENERAL SAMIGE DEL BICENTENARIO; 2011
Institución organizadora:
SAMIGE
Resumen:
The Rcs phosphorelay system is a complex two component system that consist of the sensor protein RcsC, the response regulator RcsB and the phosphorelation intermediary RcsD. Even when the signal remains unknown, several conditions can activate the system such us the tolB, pmrA and rcsC11 mutants, as well as the rcsB overexpression and polymyxin B treatment. The Rcs regulatory system controls the colanic acid biosynthesis, the flagellar biosynthesis, the O-antigen chain length determinant, the cell division and osmoregulation, between others. Previously, we found that the rcsB gene is transcribed from two promoters: PrcsDB and PrcsB. The first promoter is located upstream of rcsD gene and high induced during the exponential growth phase, while the second promoter is located at the end of rcsD coding sequence and is lower induced during stationary phase. In the present study, we analyses the expression of certain genes that, according to microarray assay results, appears to be regulated by the RcsCDB system activation. We selected the dps and osmY genes, regulated during stationary growth phase and involved in the stress response, respectively. The activity of its promoters cloned into the pRS415 plasmid was measured by β-Galactosidase assays. The genes analyzed were modulated by the RcsB regulator under different RcsCDB system activation conditions. In the other hand, we observed that these new identified genes were controlled differentially when RcsB is produced by one of the two rcsB promoters. Here, we report several genes as new members of the RcsB regulon that contributes to the Rcs system study and allow us to postulated the implication of this system in others physiologically roles.