CONICET | Buscador de Institutos y Recursos Humanos

Petroleum hydrocarbons are well known by their high toxicity and recalcitrant properties. Their increasing utilizationaround worldwide led to environmental contamination. Phytoremediation using plant-associated microbeis an interesting approach for petroleum degradation and actinobacteria have a great potential for that. Forthis purpose, our study aimed to isolate, characterize, and assess the ability of endophytic actinobacteria to degradecrude petroleum, as well as to produce plant growth promoting traits. Seventeen endophytic actinobacteriawere isolated from roots of plants grown naturally in sandy contaminated soil. Among them, six isolates wereselected on the basis of their tolerance to petroleum on solid minimal medium and characterized by 16S rDNAgene sequencing. All petroleum-tolerant isolates belonged to the Streptomyces genus. Determination by crude oildegradation by gas chromatorgraph-flame ionization detector revealed that five strains could use petroleum assole carbon and energy source and the petroleum removal achieved up to 98% after 7 days of incubation. Theseisolates displayed an important role in the degradation of the n-alkanes (C⁠6-C⁠30), aromatic and polycyclic aromatichydrocarbons.All strains showed a wide range of plant growth promoting features such as siderophores, phosphate solubilization,1-aminocyclopropane-1-carboxylate deaminase, nitrogen fixation and indole-3-acetic acid production aswell as biosurfactant production. This is the first study highlighting the petroleum degradation ability and plantgrowth promoting attributes of endophytic Streptomyces. The finding suggests that the endophytic actinobacteriaisolated are promising candidates for improving phytoremediation efficiency of petroleum contaminated soil.

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