Preliminary report of assymetrical IgG in porcine serum.

GARRO, A.; GENTILE, T.; DE LEÓN, R.; KONCURAT, M.

Facultad de Medicina, UBA - Ciudad Autónoma de Buenos Aires

Taller; I Taller de Interacción Materno-Fetal: de la Fisiología a la Patología.; 2005

Centro de Estudios Farmacológicos y Botánicos, Laboratorio De Fisiopatogenia-Facultad de Medicina, UBA e Inst. de Estudios de la Inmunidad Humoral-Facultad de Farmacia y Bioquímica, UBA.

<!-- @page { margin: 2cm } P { margin-bottom: 0cm; text-align: justify } P.western { so-language: es-ES } P.ctl { font-size: 10pt } A:link { color: #0000ff } --> <!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } H1 { margin-bottom: 0.21cm } H1.western { font-family: "Arial", sans-serif; font-size: 16pt } H1.cjk { font-family: "DejaVu Sans"; font-size: 16pt } H1.ctl { font-family: "DejaVu Sans"; font-size: 16pt } --> 6-Preliminary report of assymetrical IgG in porcine serum. A.Garro1, T. Gentile2, R. De León2, M. Koncurat1. 1FCV-UNLPam, Calle 5 erq. 116, General Pico (6360), La Pampa, Argentina. 2IH-UBA, Facultad de Farmacia y Bioquímica, UBA; Buenos Aires, Argentina. The normal immune response courses with the production of precipitating immunoglobulins G. In this immune response also appears another population of antibodies of the same kind that have different immunochemical and biological behaviours, named asymmetrical IgG antibodies. Their presence in serum during human and murine pregnancy was reposted. The aim of this work was to investigate the existence of asymmetrical IgG in different stages of pregnancy and in non-pregnant porcine sera. Porcine sera of non pregnant females, ±30, ±75 and ±90 days of pregnancy and next-to-deliver females were used. Half of the samples were treated with buffer solution and the other half with Con-A. The presence of total and symmetric IgG was determined with differential ELISA technique using anti-Fc antibody of porcine Ig fixed to a solid phase and anti-IgG porcine antibody conjugated with peroxidase, then revealed with 1,2 ortofenilendiamina. The asymmetric IgG values were calculated: % of IgG joined to Con-A = 1- [(not joined IgG – total IgG) x 100]. The results were: ±30 days: 29,67%; ±75 days: 34,38%; ±90 days: 42,40%; next-to-deliver females: 54,10% and non-pregnant females: 39,80%. The percentages of asymmetrical antibodies found in next-to-deliver females sera were greater than those found in non-pregnant swine. In conclusion: during porcine pregnancy the asymmetric IgG antibodies are present and they could play a role as protector antibodies. Fuente de Finaciamiento: SeCyT -UNLPam