INVESTIGADORES
BOLLO Mariana Ines
capítulos de libros
Título:
Metabolic Regulation of the Squid Nerve Na+/Ca2+ exchanger: Recent Developments
Autor/es:
BEAUGÉ L., DIPOLO R., BOLLO M., COUSIDO A., BERBERIAN G. AND PODJARNY A
Libro:
Sodium Calcium Exchange: A Growing Spectrum of pathophysiological implications. Advances in Experimental Medicine and Biology
Editorial:
Springer Science + Business Media
Referencias:
Lugar: New York; Año: 2012; p. 1 - 14
Resumen:
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In squid,MgATP modulation of the Na+/Ca2+
exchanger requires the resence of a cytosolic protein which becomes
phosphorylated durinf the process. This factor has been recentrly identified.
Mass spectroscopy and Western blot analysis established that it is a member of
the lipocalin superfamily of lipid-binding proteins (LBP or FABP) of 132 amino
acids. We called it regulatory protein of squid nerve sodium/calcium exchanger
(ReP1-NCXSQ, access to GenBank EU981897).
ReP1-NCXSQ
was clones, expressed, and purified. Circular dichroism, far-UV, and infrared
spectroscopy suggest a secondary structure, predominantly of beta-sheets. The
tertiary structure prediction provides ten beta-sheets and two alpha-helices,
characteristic of most of LPB. Fucntional experiments showed that, to be
active, ReP1-NCXSQ must be phosphorylated by MgATP, though the action of a
kinase present in the plasma membrane. Moreover, PO4-ReP1-NCXSQ can stimulate
the exchanger in the absence of ATP. An additional crucial observatiob was
that, in proteoliposomes containing only the purified Ba+/Ca2+ exchanger,
PO4-PeP1-NCXSQ promotes activation; therefore, this upregulation has no other
requirement than a lipid membrane and the incorporated exchanger.
