IDEAUS - CENPAT   25626
INSTITUTO DE DIVERSIDAD Y EVOLUCION AUSTRAL
Unidad Ejecutora - UE
artículos
Título:
Testing repeatability, measurement error and species differentiation when using geometric morphometrics on complex shapes: A case study of Patagonian lizards of the genus Liolaemus (Squamata: Liolaemini)
Autor/es:
VRDOLJAK, JUAN; HUESA, EMILCE GUADALUPE DIAZ; MORANDO, MARIANA; ARREOLA-RAMOS, ROBERTO; AVILA, LUCIANO JAVIER; ARREOLA-RAMOS, ROBERTO; AVILA, LUCIANO JAVIER; SANCHEZ, KEVIN IMANOL; VILLAGRA, ALEJANDRO; SANCHEZ, KEVIN IMANOL; VILLAGRA, ALEJANDRO; VRDOLJAK, JUAN; HUESA, EMILCE GUADALUPE DIAZ; MORANDO, MARIANA
Revista:
BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2020 vol. 130 p. 800 - 812
ISSN:
0024-4066
Resumen:
The repeatability of findings is the key factor behind scientific reliability, and the failure to reproduce scientific findings has been termed the ?replication crisis?. Geometric morphometrics is an established tool in evolutionary biology. However, different operators (and/or different methods) could act as large sources of variation in the data obtained. Here, we investigated inter-operator error in geometric morphometric protocols on complex shapes of Liolaemus lizards, as well as measurement error in three taxa varying in their difficulty of digitalization. We also examined the potential for these protocols to discriminate among complex shapes in closely related species. We found a wide range of inter-operator error, contributing between 19.5% and 60% to the total variation. Moreover, measurement error increased with the complexity of the quantified shape. All protocols were able to discriminate between species, but the use of more landmarks did not imply better performance. We present evidence that complex shapes reduce repeatability, highlighting the need to explore different sources of variation that could lead to such low repeatability. Lastly, we suggest some recommendations to improve the repeatability and reliability of geometric morphometrics results.