IDEAUS - CENPAT   25626
INSTITUTO DE DIVERSIDAD Y EVOLUCION AUSTRAL
Unidad Ejecutora - UE
artículos
Título:
Effect of glucocorticoids on androgen biosynthesis in the testes of the toad Rhinella arenarum (Amphibia, Anura).
Autor/es:
VOLONTERI, MARÍA CLARA; REGUEIRA, ELEONORA; CZUCHLEJ, SILVIA CRISTINA; CEBALLOS, NORA RAQUEL
Revista:
Journal of Experimental Zoology Part A: Ecological and Integrative Physiology
Editorial:
John Wiley and Sons Inc.
Referencias:
Lugar: New York; Año: 2019 vol. 331 p. 17 - 26
ISSN:
2471-5638
Resumen:
In rat Leydig cells, glucocorticoids (GCs) inhibit testosterone production through the interaction with the glucocorticoid receptor (GR). However, the sensitivity of those cells to GCs is regulated by the enzyme 11β-hydroxysteroid dehydrogenase Type 1 (11β-HSD1). In the testes of the toad Rhinella arenarum, the presence of an 11β-HSD similar to type 2 and a cytosolic GR has also been described. However, there is a lack of information regarding the effects of GCs on amphibian testicular steroidogenesis. In this study, the effects of corticosterone on androgen production, and the activity of two steroidogenic enzymes in toad testes were reported. Corticosterone inhibits androgen production via the GR because the GR antagonist RU486 prevents corticosterone-induced inhibition of testosterone. Corticosterone also reduced the activity of the cytochrome P450 17-hydroxylase, C17,20-lyase (Cyp450 c17 ) without affecting the 3β-hydroxysteroid dehydrogenase/isomerase activity. This effect on Cyp450 c17 was likewise inhibited by RU486. On the other hand, corticosterone had no effect on the amount of steroidogenic acute regulator protein. These results suggest that GCs inhibit steroidogenesis in toad testes by reducing of Cyp450 c17 activity via a GR-mediated mechanism.