IPEEC - CENPAT 25619
INSTITUTO PATAGONICO PARA EL ESTUDIO DE LOS ECOSISTEMAS CONTINENTALES
Unidad Ejecutora - UE
artículos
Título:
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
Autor/es:
SOLEDAD L. SAAVEDRA; GUILLERMINA FORNO; ROSA ERRA-BALSELLS; SILVIA A. CAMPERI; MARÍA C. MARTÍNEZ-CERON; MARÍA B. BOSCO; FERNANDO ALBERICIO; SILVANA L. GIUDICESSI; MARIELA M. MARANI; OSVALDO CASCONE
Revista:
BIOTECHNOLOGY PROGRESS
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2018 vol. 34 p. 999 - 1005
ISSN:
8756-7938
Resumen:
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5.
