Implementation of Fisher ratio analysis for metabolite discovery in pacu fish using comprehensive two-dimensional gas chromatography with mass spectrometry

SARAH PREBIHALO; CELINA M. MONZÓN; SCHÖNEICH, SONIA; SYNOVEC, ROBERT

Houston

Conferencia; ASMS (American Society for Mass Spectrometry) Annual Conference; 2020

ASMS (American Society for Mass Spectrometry)

Tile-based Fisher ratio (F-ratio) analysis is used to discover and quantify 32 metabolites in Argentinian pacu fish, using data collected by comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC×GC-TOFMS) via a LECO Pegasus BT 4D instrument. The experimental design for F-ratio analysis has two sample classes: pacu fish (class one), and pacu fish spiked with a 32 metabolite standard (class two). Pacu fish are often farmed along with rice crops, which introduces sources of contamination that may alter the fish metabolome, making such a study challenging. To address this challenge, this GC×GC-TOFMS instrument provides outstanding separation peak capacity and detection sensitivity. Workflow principles to optimally apply tile-based F-ratio analysis are also presented.