IQUIBA-NEA   25617
INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Comparative characterization and synergism between two basic phospholipases A2 from Bothrops diporus snake venom
Autor/es:
BUSTILLO, SOLEDAD; ANGULO, YAMILETH; FERNÁNDEZ, JULIAN; LEIVA, LAURA; CHAVES-ARAYA, STEPHANIE; LOMONTE, BRUNO
Lugar:
Buenos Aires
Reunión:
Congreso; 20th World Congress of the International Society on Toxinology; 2019
Institución organizadora:
International Society on Toxinology
Resumen:
The vast majority of snakebite envenomings in northeastern Argentina are caused by Bothrops diporus (yarará chica), a viperid distributed from southwestern Brazil through Paraguay to central Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phospholipases A2 (PLA2-I and PLA2-II) were isolated from this venom, and their pathological effects upon murine skeletal muscle and myogenic cells in culture were analyzed. Partial amino acid sequencing showed that PLA2-I and PLA2-II are Asp49 and Lys49 PLA2s, respectively. In agreement with this, PLA2-I showed PLA2 activity, whereas PLA2-II did not. Functional assays revealed differences in their myotoxicity, cytotoxicity, and anti-adhesion activity, and in the ability to inhibit cell migration, all of which were greater for the Lys49 variant. Native electrophoresis showed that PLA2-I was less basic than PLA2-II. The two proteins act synergistically to affect the integrity of C2C12 myogenic cells, providing a further example of the concerted action of coexisting snake venom components