Impaired cell viability and functionality of macrophages cell culture after incubation with plasma of patinets with hemodialysis

HUESPE L; MIZDRAJI C.; FERRINI L; TING L.; AGUIRRE V; LAZZERI S; AUCHTER MÓNICA; RODRIGUEZ JP

San Pablo

Congreso; XXIV Congreso Brassileiro de Medicina Intensiva; 2019

SOCIEDAD BRASILEIRA DE TERAPIA INTENSIVA

ABSTRACT EP 362 Vol 31 (Supl1) 2019 ppS-163Objective: The presence of oxidative stress contributes to anincrease in failure to adapt to dialysis, greater cardiovascularcompromise and premature mortality in patients withchronic kidney disease. We explore the alteration andfunctionality of macrophages after plasma incubation ofhemodialysis patients as a mechanism of renal impairmentleading to CKD.Methods: Patients were studied in HD where demographicdata, etiology of renal disease, treatment time in years,antihypertensive mediation and determinations Nt-ProBnp,TnT, Albumin, Ferritin, CV Evaluation. Laboratory studieswas determined in the mean weekly time and plasma wasseparated to perform incubation in macrophage cell cultures.The biochemical test were analyzed in quadruplicate and invitro test using macrophages cell line.Results: 30 p were studied with chronic kidney disease,Age 38-83 years X 61.5, HD treatment 1-20 y, mean 6.8years, 10 p DBT, 15 HTA, 22 with HTA medication, Nt-ProBnp 992-3500 means 11118 preHd, means 10616postHd, normal TnT, Hb 7.2-12.2 means 10.3, Albuminmeans 3.83. Cell cultures assay showed different degrees ofimpairment 4 and 24 hs were incubated and morphologicalanalysis for monolayers to evaluate changes compatible withcell damaged in all cases.Conclusion: The evidence indicates that oxidativestress in patients with chronic kidney disease is not onlylimited to the kidneys and may contribute to an increasein cardiovascular disease, alteration in adequacy todialysis treatment and cell arrest in macrophage culturescharacteristic of the inflammatory process non-infectiousrelated to hemodialysis