IQUIBA-NEA   25617
INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Unidad Ejecutora - UE
artículos
Título:
Anti-snake venom activities of Casearia sylvestris extracts.
Autor/es:
MATÍAS DAMIÁN DUQUE DE ARCE; ANA M. TORRES; EDUARDO DELLACASSA; ANA MELISSA GONZALEZ MIRAGLIOTTA
Revista:
MEDICINA (BUENOS AIRES)
Editorial:
MEDICINA (BUENOS AIRES)
Referencias:
Lugar: Buenos Aires; Año: 2017 vol. 77 p. 605 - 605
ISSN:
0025-7680
Resumen:
Casearia sylvestris Sw. (burro-kaa, guaçatonga), has ethnopharmacological antecedents as ophidian antivenom in several regions, inhibiting the hemorrhagic, edematogenic, proteolytic and coagulant activities of Bothrops sp venom. In this study, we evaluated the activity of native plants extraxts against B. diporus venom in order to verify the ethnopharmacological knowledge from the northeast region of Argentina. Aerial parts of C. sylvestris were collected in Corrientes at two different vegetative stages: autumn (I) and spring (II). The material was dried and powdered in order to prepare aqueous, ethanolic and hexanic extracts. The screening of the extracts antisnake venom activity was performed by SDS-PAGE electrophoresis. The coagulant activity neutralization was evaluated by recalcification time of citrated plasma with a Col1 coagulometer. Inhibition of the indirect hemolytic activity was evaluated through on agarose-blood-phosphatidylcoline gel plates and the inhibition of the proteolytic activity by SDS-PAGE with casein. Phytochemical analysis was performed in order to identify chemical groups of compounds. The results from SDS-PAGE showed the ethanol extract as the most active against B. diporus venom, independently of the vegetative state of the plant. The ethanol extract inhibited 54% (II) and 60% (I) of coagulant activity (venom-extract ratio, 1:30); 50% (II) and 44% (I) of indirect hemolytic activity (1:40) and 100% (I) and (II) of proteolytic activity (1:30) of venom. The demonstrated phenols, anthraquinones, alkaloids, steroids and tannins were found as components of the active extract. In conclusion, we were able to demonstrate that the ethanolic extracts of C. sylvestris leaves possess in vitro antisnake activity against B. diporus venom supporting its ethnopharmacological antecedents. Further research is required to identify the single components responsible for this activity and, so, the best way for their administration in ophidian accidents.