IQUIBA-NEA 25617
INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Unidad Ejecutora - UE
artículos
Título:
APOPTOSIS INDUCED BY A SNAKE VENOM METALLOPROTEASE FROM BOTHROPS ALTERNATUS VENOM IN C2C12 MUSCLE CELLS
Autor/es:
VAN DE VELDE, A; MATZNER, VERÓNICA; LEIVA, LAURA; BUSTILLO, SOLEDAD; GAY, CLAUDIA C.
Revista:
APOPTOSIS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017 vol. 22 p. 491 - 501
ISSN:
1360-8185
Resumen:
In this study, the apoptosis inducing effectsof baltergin as well as its influence on cell adhesion andmigration on muscles cells in vitro were studied. Morphologicalanalysis made by scanning electron and phasecontrast microscopy demonstrated typical futures of programmedcell death, apoptosis. This mechanism was confirmedby fluorescence staining, molecular analysis ofendonuclease activity and increased mRNA expressionlevel of two representative genes (p53 and bax). On theother hand, baltergin exert an inhibition effect on myoblastcell adhesion and migration in vitro probably througha mechanism that involves the interaction of this enzymewith cell integrins. In conclusion, our results suggest thatthe absence of appropriate extracellular matrix contactstriggers anoikis. Therefore, this is the first report that demonstratedthe mechanism of programmed cell death triggeredby baltergin, a PIII metalloprotease isolated fromBothrops alternatus venom, in a myoblast cell line.
