Effect of trehalose and spermine on plasma membrane fluidity after ram sperm cryopreservation

LÓPEZ ARMENGOL, MARÍA FERNANDA; VENTURINO, ANDRÉS; AMBROSI, CLAUDIA PAMELA; AISEN, EDUARDO GABRIEL

Tours

Congreso; 18th International Congress on Animal Reproduction (ICAR); 2016

ICAR

Fluidity of sperm head plasma membrane (PM) is modified stepwiseduring maturation, capacitation and acrosomal reaction (AR). It increases atthe acrosomal caput region during capacitation. After AR, the fluidity of thePM overlying the equatorial segment also increases, and PM becomesfusogenic. Capacitation and ??cryocapacitation?? are not equivalent processes,but it is well known that the proportion of cells exhibiting PM lipid disorderand phospholipid scrambling increases with both capacitation andcryopreservation [1]. Trehalose (Tre) stabilizes the PM by interacting with thepolar head groups of phospholipids, thus increasing its fluidity [2]. Spermine(Spm) is a polyamine with antioxidant and stabilizing properties [3]. Finally,the study of M540 staining by means of fluorescence microscope has beenreported a long time ago. It allows the differentiation of different PM fluiditypatterns (4).