CITAAC   25595
CENTRO DE INVESTIGACIONES EN TOXICOLOGIA AMBIENTAL Y AGROBIOTECNOLOGIA DEL COMAHUE
Unidad Ejecutora - UE
artículos
Título:
B-esterase determination and organophosphate insecticide inhibitory effects in JEG-3 trophoblasts.
Autor/es:
ESPINOZA, MARLON; ROSENBAUM, ENRIQUE; RIVERO-OSIMANI VALERIA; GUIÑAZÚ NATALIA; SANCHEZ VICTORIA GUADALUPE; ESPINOZA, MARLON; ROSENBAUM, ENRIQUE; RIVERO-OSIMANI VALERIA; GUIÑAZÚ NATALIA; SANCHEZ VICTORIA GUADALUPE
Revista:
TOXICOLOGY IN VITRO : AN INTERNATIONAL JOURNAL PUBLISHED IN ASSOCIATION WITH BIBRA.
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2016 p. 190 - 197
ISSN:
0887-2333
Resumen:
The placenta and trophoblasts express several B-esterases. This family includes acethylcholinesterase (AChE), carboxylesterase (CES) and butyrylcholinesterase (BChE), which are important targets of organophosphate insecticide (OP) toxicity. To better understand OP effects on trophoblasts, B-esterase basal activity and kinetic behavior were studied in JEG-3 choriocarcinoma cell cultures. Effects of the OP azinphos-methyl (Am) and chlorpyrifos (Cp) on cellular enzyme activity were also evaluated. JEG-3 cells showed measurable activity levels of AChE and CES, while BChE was undetected. Recorded Km for AChE and CES were 0.33 and 0.26 mM respectively. Native gel electrophoresis and RT-PCR analysis demonstrated CES1 and CES2 isoform expression. Cells exposed for 4 and 24 h to the OP Am or Cp, showed a differential CES and AChE inhibition profiles. Am inhibited CES and AChE at 4 h treatment while Cp showed the highest inhibition profile at 24 h. Interestingly, both insecticides differentially affected CES1 and CES2 activities. Results demonstrated that JEG-3 trophoblasts express AChE, CES1 and CES2. B-esterase enzymes were inhibited by in vitro OP exposure, indicating that JEG-3 cells metabolization capabilities include phase I enzymes, able to bioactivate OP. In addition, since CES enzymes are important for medicinal drug activation/deactivation, OP exposure may interfere with trophoblast CES metabolization, probably being relevant in a co-exposure scenario during pregnancy.