IICAR   25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Preliminary Studies of the Role of TGS1 in sRNA Methylation and Splicing during Paspalum Notatum Reproductive Development
Autor/es:
PODIO, MARICEL; COLONO, CAROLINA MARTA; PESSINO, SILVINA CLAUDIA
Reunión:
Seminario; 7th biennial Seminars on Advances in Apomixis Research; 2020
Institución organizadora:
CERZOS, Bahía Blanca
Resumen:
Paspalum notatum is one of the main apomixis grass models. Previous transcriptomeanalysis further validated by mRNA in situ hybridization revealed that the gene TGS1(encoding the RNA methyltransferase TRIMETILGUANOSINE SYNTHASE 1) is repressedin ovules of apomictic P. notatum plants. In animals and/or yeasts, TGS1 promotes theprocessing several RNA types and also acts as a transcriptional co-activator. Recently,we reported that silencing of TGS1 causes the emergence of foliar trichomes and supernumeraryaposporous-like embryo sacs in P. notatum, which led to its classification as adevelopmental repressor. Our objective here was to analyze whether TGS1 influences RNAprocessing in this species. Three-hundred and sixteen (316) RNA transcripts differentiallyexpressed in flowers of apomictic and sexual plants were selected to examine the existenceof putative splice variants. Using qRT-PCR analysis, we confirmed that an unprocessed splicing variant of one of them (CHLOROPHYLL A-B BINDING PROTEIN 1B-21, renamedCHLORO) is less represented in apomictic plants at a statistically significant level. The sameunprocessed splicing variant also diminished in antisense tgs1 sexual lines. Moreover,since we identified seven miRNAs upregulated in floral sRNA libraries of sexual P. notatumplants, we are testing their representations in the same tgs1 antisense lines. Our conclusionis that TGS1 influences the splicing of CHLORO, a component of the photosynthesis lightharvestingthe photosynthesis antenna, which might relate with the role of this gene as leaftrichomes formation repressor. The function of TGS1 in miRNA regulation remains unclear.