IICAR   25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differential gene expression at different fruit ripening stages in two tomato near isogenic lines differing in fruit shelf life
Autor/es:
RODRÍGUEZ, G.R.; DI GIACOMO, M; PEREIRA DA COSTA, J. H.
Lugar:
Foz do Iguazú
Reunión:
Congreso; International Congress of Genetics; 2018
Resumen:
Tomato (Solanum lycopersicum L.) has emerged as a model to study fruit ripening and to investigate the molecular bases of fruit quality traits, such as shelf life. The aim was to study the differential gene expression at mature green (MG) and red ripe (RR) ripening stage in two near isogenic lines of tomato (NILs) differing in fruit shelf life. The NILs have introgressed chromosomal regions of the wild accession LA722 (S. pimpinellifolium L.) into the genetic background of Caimanta (CAI, S. lycopersicum). NIL115-PP and NIL115-CC have wild and cultivated segments around marker SSR155 respectively, associated with long shelf life. Standard protocols for cDNA-AFLP were applied using three different primer combinations. A 52% and 42% of polymorphism were found when the NILs were compared between them at each ripening stage. Exclusive transcript derived fragments (TDFs) were detected for genotypes. Total number of TDFs for NIL115-PP was higher than for NIL115-CC, at both MG and RR stages. When two ripening stages were compared within each genotype, the 91% and 80% out of TDFs (NIL115-PP and NIL115-CC, respectively) displayed altered expression patterns during ripening. The number of exclusive TDFs at MG was higher than RR in both genotypes. We conclude that several genes with putative role in fruit shelf life are differentially expressed between these tomato NILs. On the other hand, different expression pattern was revelled during fruit ripening.