IICAR   25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Propagation Fidelity and Kinship of Tomato Varieties ‘UC 82’ and ‘M82’ Revealed by Analysis of Sequence Variation
Autor/es:
CAMBIASO, VLADIMIR; RODRÍGUEZ, GUSTAVO RUBÉN; FRANCIS, DAVID MERRILL
Revista:
Agronomy
Editorial:
MDPI
Referencias:
Año: 2020 vol. 10
Resumen:
Plantvarieties are named and released based on distinct, unique and stablecharacteristicsbutmaybemaintainedseparatelybygenebanksorstockcentersunderseparateaccessionidentificationnumbers. Genetic heterogeneity of the original variety, genetic drift, failure toexclude cross pollination, and propagation error may erode the integrity ofgenetic resources. The availability of resequencing and genotyping data forduplicate samples enables an analysis to clarify the relationship betweenspecific varieties or independently curated accessions of the same variety whilealso assessing the fidelity of germplasm maintenance. We accessed both SingleNucleotide Polymorphism (SNP) array genotypes and resequencing data for two importanttomato varieties ?UC82? and ?M82? that have been maintained as separateaccessions in collections as important resources for the research and breedingcommunities. Our analysis of these data suggests that polymorphism rates fromresequencing of cultivated tomato are overestimated in the literature due toheterozygous calls caused by either sequence error or coalignment of repetitivesequences. We defined a set of 32,352 robust SNPs from a file containing data forall samples and we compared the distribution of data with SNPs called from agenotyping array. For both analyses, intravariety variation was found inhaplotype blocks, with the same haplotypes identified using SNPs detected fromarray and sequence data. The distribution pattern of variation across the entiregenome sequence was similar for both ?UC82? and ?M82?. Overall, the differences betweendistinct accessions of a variety were nearly as great as the differences between?UC 82?and ?M82?. The similarities between ?UC82? and ?M82? range from 99.33%to 99.74% and are highly consistent with a common pedigree and shared selectionfrom partially inbred progeny. The data also suggest that these tomato geneticresources have been propagated with high fidelity.