IICAR   25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Construction and validation of core collections in Pisum sp. using different methodologies
Autor/es:
GATTI, I; COINTRY E; ESPÓSITO, M. A
Revista:
LEGUME RESEARCH
Editorial:
AGRICULTURAL RESEARCH COMMUNICATION CENTRE
Referencias:
Año: 2019 vol. 46 p. 743 - 749
ISSN:
0250-5371
Resumen:
Core collections contributeto make conservation expenses more efficient and facilitate a betterutilization of accessions in breeding programs. Eighty-five accessions fromthe working collection of Pisumgermplasm belonging to cultivated species and subspecies were evaluated during2015 and 2016 in the College of Agricultural Sciences, Rosario National University. Phenotypicvalues of 12 morphological traits were measured and best linear unbiased prediction(BLUP) of the adjusted means or genotypic values for these traits wascalculated. Molecular characterization was performed assaying a total of 15 SSR primercombinations and 25 SRAP primer combinations on all accessions. With all the datacollected, four Cluster Analysis were performed (phenotypic values,genotypic values, molecular markers and consensus) to divide theaccessions into groups with similar characteristics. Four strategies todetermine the number of accessions selected from each group (constant,logarithmic, proportional and maximization strategies) were applied toconstruct 16 core collections. Validation of the corecollections were performed analyzing the parameters Mean difference percentage(MD), Variance difference percentage (VD), Coincidence rate of range(CR) Variable rate of coefficient of variation (VR), Shannon diversity index(SW) and the taxonomy coverage (TC). Consideringall validation parameters together, the logarithmic strategy with genotypicvalues data was the best strategy, while the worst strategy was theproportional strategy with molecular marker data