IICAR   25568
INSTITUTO DE INVESTIGACIONES EN CIENCIAS AGRARIAS DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Repetitive sequences in Eragrostis curvula cDNA EST libraries obtained from genotypes with different ploidy
Autor/es:
ROMERO J; SELVA JP; PESSINO SC; ECHENIQUE V; GARBUS I
Revista:
BIOLOGIA PLANTARUM
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2016 vol. 60 p. 55 - 67
ISSN:
0006-3134
Resumen:
Eragrostis curvula (Schrad) Nees (weeping lovegrass) represents important cultivated forage in semiarid regions, andthe most useful cultivars are tetraploid and reproduce by pseudogamous diplosporous apomixis. We previouslyproduced a series of genetically related E. curvula lines that provide a suitable system for the identification of gene(s)involved in diplosporous apomixis and ploidy, including a natural apomictic tetraploid (T), a diploid sexual line (D),and a tetraploid sexual plant (C). A collection of expressed sequence tags (ESTs) was generated from cDNA librariesobtained from panicles of the D, T, and C, and leaves of the T. The present study aimed to analyze the repetitive contentof these four cDNA libraries and further identify and characterize transposable element (TE)-related ESTs. Repetitivesequences were identified through the interface RepeatMasker (RM) using the database Repbase Update and furtherclassification of TEs was performed manually from the RM output. The different contribution of ESTs with identity toTEs among libraries was further evaluated, and such differences were validated through RT-qPCR. We found that thepercentage of repetitive content in the leaf cDNA library was almost double than in inflorescence libraries, withretrotransposons contributing mostly in all libraries. The expression of TE-related ESTs was compared in cDNAsamples extracted from D, T, and C leaves or inflorescences revealing that seven mRNAs containing MuDR-like DNAtransposons, Gypsy-like, and Copia-like retrotransposons were differentially represented according to tissue,reproductive mode, or ploidy. The euploid series of Eragrostis curvula is a useful model to the study of epigenomicchanges produced after changes in ploidy. The present work constitutes the first detailed report on repetitive sequencesof Eragrostis curvula at the transcriptome level.