ICC   25427
INSTITUTO DE INVESTIGACION EN CIENCIAS DE LA COMPUTACION
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The concept of electroporation energy in electroporation-based models
Autor/es:
MARINO, M.; TURJANSKI, P.; MINOTTI, FERNANDO; MÁRQUEZ, ADRIANA; GARABALINO MA; TELLADO, M.; SANTA CRUZ, GUSTAVO; MAGLIETTI, FELIPE; OLAIZ, NAHUEL; E LUJAN; ZUCCO, STELLA; MICHINSKI S; MARSHALL, GUILLERMO
Lugar:
Norfolk
Reunión:
Congreso; 2nd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies; 2017
Resumen:
Electroporation (EP) based theoretical models have been discussed since long. In particular, those based on the solution of the nonlinear Laplaceequation using a variable electric conductivity coefficient expressed as a function of electric field, temperature, and other variables, had been widely used. Here we introduce the concept of electroporation energy and its use in the solution of the nonlinear Laplace equation in which the tissue electric conductivity is made dependent of the electric field, temperature, and permeabilization. The permeabilization function links the tissue and membrane scales through the electroporation energy, that is, the energy absorbed by the membrane to change its state. The total electric energy pumped into the system is mainly distributed among electroporation, thermal and mechanical energies. In the model, the total energy is calculated with the applied voltage between electrodes,total electric current and pulse time. The thermal energy and pH effects are calculated via the Bioheat equation and the Nernst-Planck equations describing ion transport, respectively. Electroporation energy can be computed via the difference between total energy and thermal energy. The model is calibrated via experimental measurements in potato samples. Here electric current, voltage pulses and time are recorded, the electroporated area is obtained through image processing of blackened potato area. Temperature changes are measured with an infrared camera and pH changes with basic and acid pH indicators. The model presented is able to predict the space-time evolution of the electroporation front, electric current, pH fronts and temperature variations over abroad range of applied electric energies, with an excellent agreement with experiments. These results suggest that the model based on the concept of electroporation energy is appropriate for predicting EP based protocols. The non-thermal property of EP based protocols lies in the fact that a large portion of the total energy pumped into the system goes into the electroporation energy.