Inhibition of cardiac hypertrophy and normalization of cardiac contractile response in experimental hypertension disparate results in rats

GOMEZ LLAMBI H; M DONATO; B BUCHHOLZ; G OTTAVIANO; A MÜLLER; GELPI R; M OTERO LOSADA; J MILEI

Londres

Congreso; 22th European Meeting of Hypertension and Cardiovascular Protection; 2012

European Society of Hypertension

INHIBITION OF CARDIAC HYPERTROPHY AND NORMALIZATION OF CARDIAC CONTRACTILE RESPONSE IN EXPERIMENTAL HYPERTENSION. DISPARATE RESULTS IN RATS Autor/es: GOMEZ-LLAMBI HERNAN; DONATO M; BUCHHOLZ B; OTTAVIANO G; MULLER A; GELPI R; OTERO-LOSADA M; MILEI J Lugar: London Reunión: Congreso; 22nd. European Meeting on Hypertension and Cardiovascular Protection; 2012 Institución organizadora: European Society of Hypertension Resumen:" Objective: To evaluate if chronic antihypertensive treatment inhibits the left ventricular hypertrophy (LVH) and reverses the impairment in cardiac response to beta adrenergic stirnuIation. Design and Method: MaIe SpontaneousIy Hypertensive Rats (SHR), 8 weeks oId, were treated with either Iosartan 30mg/kg (L), hydralazine llmg/kg (H), rosuvastatin 10 mg/kg (R), (n=18 each group) or carvedilol20 mg/kg (C), (n=16) per os (tap water) for 16 months. Untreated SHR and Wistar Kyoto (WKY) groups (n=18 each) were included as controls. Systolic bIood pressure (SBP) was periodically measured (tail cuff plethysmography). At the end of treatment the animals were weighed (body weight, BW) and euthanasia was practiced under pentobarbital anesthesia. Half of the hearts were mounted in a Langendhorf preparation. Left ventricular end diastolic pressure (LVEDP), developed ventricular pressure (LVDP), the first derivate of developed pressure (DP/DT +), the first derivate of decrement of developed pressure (dp/dt-) and TAU, as a relaxation index, were recorded before and after isoproterenoI (ISO) (10-9M,1O-7M and 1O-5M). The other half of the hearts were weighted and used for conventional histology. Myocyte diameters (MD) were measured. Results: High SBP values were not associated with LVH in C group. Basal contractile parameters were depressed in SHR, R and C vs. WKY and L. The ISO at 1O-5M was similar in WKY and L but remain depressed in C, R and SHR. *p