IPROBYQ   25157
INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Application of thymine-based copolymers in the quantification of organic pollutants in water by sensitized fluorescence
Autor/es:
BOSCHETTI, CARLOS E.; LEDESMA, JULIETA; BORTOLATO, SANTIAGO A.; MARTINO, DEBORA M.
Revista:
MICROCHEMICAL JOURNAL
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2020 vol. 155
ISSN:
0026-265X
Resumen:
Bio-inspired copolymers based on thymine can interact with certain organic molecules and therefore they can beused as organized media or as optical sensors for analytical determinations. Particularly, the thymine-basedcopolymers have a high affinity for benzo(a)pyrene (BaP) and pyrene (Pyr), two main persistent organic pollutantsin water. In the present work, such interaction is applied for the development of a simple analyticalmethod based on fluorescence enhancement of BaP and Pyr. Since BaP and Pyr fluorescence spectra are stronglyoverlapped, a multivariate analysis of the data is proposed. The selectivity of the method was achieved through aparallel factors analysis (PARAFAC) of excitation-emission fluorescence matrices. The method performance wastested by simultaneous determination of BaP and Pyr in water samples, without pre-concentration steps or fullchromatographic resolution. Studies of recovery for both analytes added to tap and surface waters were done,obtaining results in the order of 87?115%. The analysis was performed in the presence of additional specieswhose signals overlap with those of the analytes. In all cases, the calculated analytical figures of merit werecompared with those obtained by the reference chromatographic method. In addition, a comparison of ourmethod with the reference one is made based on the calculation of their respective eco-scales. The quality of theachieved results indicates that a second-order advantage was reached, while our method represents a greeneralternative than the reference chromatographic methodology.