IPROBYQ   25157
INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Design and optimization of an alternative chymotrypsin purification method by adsorption onto non-soluble alginate–carrageenan bed
Autor/es:
GASTÓN, KNOBEL; BÁRBARA, BOSIO; GUILLERMO, PICÓ; EMILIA, BRASSESCO MARÍA; NADIA, WOITOVICH VALETTI
Revista:
POLYMER BULLETIN
Editorial:
SPRINGER
Referencias:
Año: 2020
ISSN:
0170-0839
Resumen:
In the present work, we have developed a new protocol for the purification of chymotrypsin using adsorption onto non-soluble alginate?carrageenan bed. In the first instance, an experimental statistical design was used to screen which parameters are significant in the adsorption process. The variables tested were pH, temperature, and medium?s ionic strength. Significant statistical differences (p