Platform design for extraction and isolation of Bromelain: Complexformation and precipitation with carrageenan

NADIA WOITOVICH VALETTI; JOSÉ A. TEIXEIRA; DÉBORA A. CAMPOS; LORENZO M. PASTRANA-CASTRO; GUILLERMO PICÓ; ANA OLIVEIRA; MARIA M PINTADO

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017 vol. 54 p. 156 - 161

1359-5113

The main objective of this work was to investigate for the first time the molecular mechanism of com-plex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strongpolyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. TheBromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility wasdependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH waschanged and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism forthe formation of non-soluble complex. The formation of the carrageenan-bromelain complex increasedin 8◦C the enzyme thermal stability, while its biological activity was not modified. The amount of totalenzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85?90%.