Production of alpha-amylase from Aspergillus oryzae for severalindustrial applications in a single step

MARÍA CECILIA PORFIRI; DIANA ROMANINI; BEATRIZ FARRUGGIA; ESTEBAN MILATICH

Journal of Chromatography B

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 1022 p. 87 - 92

1570-0232

tA one-step method as a strategy of alpha-amylase concentration and purification was developed in thiswork. This methodology requires the use of a very low concentration of biodegradable polyelectrolyte(Eudragit®E-PO) and represents a low cost, fast, easy to scale up and non-polluting technology. Besides,this methodology allows recycling the polymer after precipitation.The formation of reversible soluble/insoluble complexes between alpha-amylase and the polymerEudragit®E-PO was studied, and their precipitation in selected conditions was applied with biosep-aration purposes. Turbidimetric assays allowed to determine the pH range where the complexes areinsoluble (4.50-7.00); pH 5.50 yielded the highest turbidity of the system. The presence of NaCl (0.05 M)in the medium totally dissociates the protein-polymer complexes.When the adequate concentration of polymer was added under these conditions to a liquid culture ofAspergillus oryzae, purification factors of alpha-amylase up to 7.43 and recoveries of 88% were obtained ina simple step without previous clarification. These results demonstrate that this methodology is suitablefor the concentration and production of alpha-amylase from this source and could be applied at thebeginning of downstream processing.© 2016 Published by Elsevier B.V.