ANALYSIS OF THE EOSINOPHIL-CHEMOATTRACTANT CYTOKINE PRODUCTION IN INTESTINAL TISSUES OF PATIENTS WITH FOOD ALLERGY

JULIÁN VACCARO; LUCIANA GUZMAN; VIVIANA BERNEDO; KARINA EVA CANZIANI; EUGENIA MARGARITA ALTAMIRANO; MARCELA GARCÍA; GUILLERMO DOCENA; IVANNA ROLNY; MARÍA TERESA GONZÁLEZ VILLAR; CECILIA MUGLIA

Mar del Plata

Congreso; Reunión conjunta de sociedades de biociencias; 2018

SAI, SAIC, SAFIS.

Juvenile polyps (JP) are common in pediatric patients with milk allergyand lead to rectal bleeding and diarrhea. We have previouslycharacterized the allergic inflammatory cell infiltrate of the polyp tissue,and here we aimed to investigate the origin of the high frequencyof eosinophils, which may be responsible for tissue remodeling.Biological intestinal samples were obtained by colonoscopy in 7 patients,along with a biopsy of the surrounding tissue (SCT), as acontrol sample. The production of eotaxin-3 (CCL26) and RANTES,chemokines responsible for eosinophil homing, were assessed byqPCR and confocal microscopy in lamina propria and the epithelialcompartment. In addition, Caco-2 and HMEC-1 cell lines were employedto study the induction of chemokine production. Cells werestimulated with IFN-γ (10ng/ml) and IL-13 (10ng/ml), and the activationof STAT-3 and expression of chemokines were analyzed byimmunoblotting and qPCR, respectively.We found a significant increase of eosinophils and eotaxin-3 expressionin polyps compared to SCT (p