IIFP   25103
INSTITUTO DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Osteocyte alterations contribute to bone pathology in Gaucher disease
Autor/es:
ORMAZABAL MAXIMILIANO; MUCCI JM; CRIVARO A; BONDAR C; ROZENFELD PA
Lugar:
Zaragoza
Reunión:
Congreso; EWGGD; 2016
Resumen:
The aim of our work was to evaluate the involvement of osteocytes in the bone pathology of Gaucher disease. The study was performed with an in vitro model using the MLO-Y4 osteocyte cell line treated with CBE at different time points. The osteoclastogenic potential of conditioned media (CM) from CBE treated ostecytes was evaluated by osteoclast differentiation assays and RANKL levels as evaluated by immunofluorescence. Connexin 43 expression was evaluated by qPCR and apoptosis was studied by both Annexin V and TUNEL stainings. To evidence a possible mechanism on osteoclast differentiation, osteoclastogenesis assays were performed with the apoptotic body fraction of CM and the supernatant fraction of the CM separated by centrifugation, in the presence of OPG. The CM from CBE treated ostecytes induced higher levels of osteoclast differentiation (p