IIFP   25103
INSTITUTO DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of in vivo effects of p31-43 gliadin peptide in a murine model of enteropathy
Autor/es:
LIC. MARÍA FLORENCIA GOMEZ CASTRO; DRA. CARASI PAULA; DRA. ROMINA ELIZABETH ARAYA; LIC. FEDERICO PEREZ
Lugar:
Buenos Aires
Reunión:
Encuentro; 1st LASID -SAI-FAIC Meeting; 2015
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
Celiac Disease (CD) is a high prevalence chronic gastrointestinal disease. Villus atrophy,crypt hyperplasia, and lymphocytic infiltration are the characteristics findings in duodenal mucosa in active CD. Gluten-specific IFNγ producing T cells are abundant in the mucosa. P31-43 gliadin is themost studied model peptide which has toxic effects but is not able to induce an adaptive immune response. In previous studies, we showed the toxic effects of intraluminally delivered p31-43 in a murine model of enteropathy. Receptor and signaling pathways involved in this mechanism are unknown.Our aim was to investigate the in vivo effects of p31-43 in the small intestinal mucosa. By microsurgery in C57BL6 mice, P31-43, two peptides derived from p31-43, one with inverted sequence and another with random sequence, and a not related peptide used as control, were intraluminally administrated (100 μl, 10 μg/ml). Mice were sacrificed after 4 or 16 hs post-treatment and small intestine samples were collected and histologically evaluated. MyD88 and IFNAR KO micewere assessed as well. We found that among all the peptides studied only the intraluminal administration of p31-43 induced histological changes in the small intestinal mucosa characterized by decreased Villi/Crypt ratio(p