Characterization of the mucosal infiltrate of colorectal polyps in children with cow's milk allergy

CANZIANI, KARINA EVA; BALCARCE, NORMA; GUZMAN, LUCIANA; NANFITO, GABRIELA; ALTAMIRANO, EUGENIA; GARCÍA, MARCELA; ZANUZZI, CAROLINA; DE LA FUENTE, MARJORIE; GLAUBEN LANDSKRON; DÍAZ JIMENEZ, DAVID; HERMOSO RAMELLO, MARCELA; MUGLIA, CECILIA; DOCENA, GUILLERMO

Buenos Aires

Congreso; Imeeting LASID-FAIC-SAI 2015; 2015

LASID-FAIC-SAI

Cow milk´s allergy is the most common food allergy. Preliminary studies showed that 70% of patients older than 1 yo, with rectal bleeding and nontumoral colorectal polyps, showed high levels of serum milk-specific IgE. In this study we aimed to characterize the mucosa of polyps.Resected polyps and surrounding mucosa were studied (n=30) by histopathology (H&E), inmunofluorescence (confocal microscopy) and RT-qPCR for several immune markers. In addition, cytokines were assessed by CBA and ELISA, and peripheral and mucosal T cells were isolated and cloned.We found that in 25 patients with increased serum specific IgE levels, the stroma of polyps showed a cellular infiltrate composed of eosinophils, mast cells, IgE secreting plasma cells, ST2+ and IL-33+ cells. Colonic sorrounding tissue showed fewer frequencies of IgE+ cells (30±15 cells vs 2±1 polyps vs mucosa). Besides, we found a significant higher expression of il-4, il-33 and st2s (P