Role of galectin-1 on intestinal epithelial cells in inflammatory settings

MUGLIA C.I.; PAPA GOBBI, R; SMALDINI, P; ORSINI DELGADO, L; SAMBUELLI, A.; ROCCA, A; TOSCANO, M.; RABINOVICH, G.; DOCENA, G.

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting II French-Argentinean Immunology Meeting; 2015

Sociedad Latinoamericana de Inmunodeficiencias, Sociedad Franco-Argentina de Inmunología, Sociedad Argentina de Inmunología

Galectins play key roles in cell survival and in inflammation. Here analyzed the effect of galectin-1 (Gal-1) on function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel disease (IBD) were incubated with different pro-inflammatory cytokines and Gal-1 binding by flow cytometry, secretion of homeostatic factors by ELISA and viability (AnnexinV/PI, caspase activity and Bcl family members) were assessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 binding and modulation of IECs.We found enhanced binding of Gal-1 to: murine IECs exposed to IL-1β (P