REGULATORY T CELLS INDUCED BY ACTINOMYCES BACTERIA CONTROLLED THE ALLERGIC REACTION IN A MOUSE MODEL OF FOOD ALLERGY

SMALDINI PAOLA; TREJO FERNANDO; SINGH MARAVIN; KAMPINGA JAAP; DOCENA GUILLERMO H.

Buenos Aires

Congreso; First International Congress of LASID FAIC SAI, IV Meeting LASID and LXIII Congreso Anual de la Sociedad Argentina de Inmunología; 2015

SAI

Food allergy is an immune-mediated adverse reaction to food likely due to defective regulatory circuits. In this work we aimed to modulate milk allergy in a mouse model through the oral administration of a heat-killed Actinomyces bacteria (Tsukamurella inchonensis-Ti) and to study the immunomodulatory mechanisms. Balb/c mice were sensitized with cow´s milk proteins (CMP) plus cholera toxin by gavage, and orally challenged with CMP to evidence hypersensitivity symptoms. Thereafter, heat-killed Ti was orally administered as treatment. Mice were challenged and treatment efficacy was in vivo (clinical score and cutaneous test) and in vitro (serum specific antibodies and cytokines-ELISA, and cell analysis-flow cytometry) evaluated.Clinical signs were lower in Ti-treated mice compared with milk-sensitized mice (average score-1 for Ti-Treated vs average score-3 for milk-sensitized mice; p