Suppression of the allergic reaction in a food allergy mouse model through the oral administration of heat-killed Tsukamurella inchonensis

SMALDINI PAOLA; TREJO FERNANDO; BRUNET LAURA; KAMPINGA JAAP; DOCENA GUILLERMO H.

Berlin

Congreso; Society For Mucosal Immunology ICMI 2015; 2015

SMI

Food allergy, a worldwide immune adverse reaction to certain foods, is a growing clinical concern with no approved standardized immunotherapy. In this work we aimed to modulate milk allergy in a mouse model through the oral administration of a heat-killed Actinomyces bacteria (Tsukamurella inchonensis-Ti). Balb/c mice were sensitized with cow´s milk proteins (CMP) plus cholera toxin by gavage, and orally challenged with CMP to evidence hypersensitivity. Thereafter, Ti was orally administered during two months for immunomodulation. Mice were challenged and treatment efficacy was in vivo (clinical score and cutaneous test) and in vitro (serum specific antibodies and cytokines by ELISA, and cell analysis by flow cytometry) evaluated.Clinical signs and serum specific IgE levels were lower in Ti-treated mice compared with sensitized mice (p