Dual effects of Arachidonic acid on BK channel: role of beta-1-subunit

MONCADA, MELISA; PICCININI, LUCIANO; CASTILLO, KAREN; ASUAJE, AGUSTIN; GONZÁLEZ, CARLOS; MILESI, MARIA VERÓNICA; MARTÍN, PEDRO

Bahia Blanca

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Biofísica; 2014

Sociedad Argentina de Biofísica (SAB)

Arachidonic acid (AA) is a polyunsaturated fatty acid involved in modulation of several ion channels activity. Previously, we reported that 10 µM AA activates the high conductance Ca2+ and voltage dependent K+ channel (BK) in human vascular smooth muscle cells where the α subunit of BK is expressed together with the regulatory β1-subunit (β1) [1]. In this work, we studied in depth the action mechanism of AA using patch clamp technique on BK channel heterologously expressed with or without β1. 10 µM AA activated BK only in presence of β1, changing the voltage dependence of activation (left shift on G-V curve, ΔV1/2= -55,2 mV ± 4,4; n=3; p