IIFP   25103
INSTITUTO DE ESTUDIOS INMUNOLOGICOS Y FISIOPATOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of colorrectal polyps in children with cow milk´s allergy.
Autor/es:
CANZIANI, K.; CANDIA, M; NANFITO G; BALCARCE, N; GUZMAN, L; BOROBIA P; ALTAMIRANO E; BEN R; MUGLIA C.I.; DOCENA, G.
Lugar:
Mar del Plata
Reunión:
Congreso; LXII REUNIÓN ANUAL Sociedad Argentina de Inmunología; 2014
Institución organizadora:
Sociendad Argentina de Inmunologia
Resumen:
Cow milk allergy (CMA) is the most common food allergy amongst infants, affecting 2-5 % of children. Our preliminary studies showed that 90 % of patients older than 1 year old with rectal bleeding and colorectal polyps had high levels of serum cow?s milk protein (CMP) specific IgE. Besides, when milk is avoided as treatment no recurrent polyps were observed. In this work we studied the cellular components of the benign colonic polyps that are routinely removed. Resected polyps from 20 patients were analyzed by immunohistochemistry and confocal microscopy using anti-IgE and anti-CD138 conjugated antibodies. Besides, cytokine and transcription factor expression were analyzed by RT-qPCR; and finally T cell lines were established form peripheral blood and lamina propria to study their specificity by proliferation assays (3HThymidine and CFSE). We found that 15 patients had serum CMP-specific IgE. The stroma of polyps showed a cellular infiltrate composed of mononuclear cells eosinophils, mast cells, and membrane-bound IgE cells. We confirmed the presence of IgE secreting plasma cells and IgE-bearing mast cells. Biopsies from mucosae surrounding the polyps showed a mononuclear cell infiltrate with no eosinophils and a low frequency of IgE+ cells (30 ± 15 vs 2 ± 3 cells, polyps vs mucosa). Moreover, we found a trend to a higher expression of il-13 and gata-3 in polyps than in the lamina propria outside the polyps. T cell lines were developed from PBMC (n=6) and polyps (n=2) and they showed a high proliferative responses to CMP (P