LACTATE TRANSPORT MEDIATED BY PLACENTAL AQUAPORIN 9

SIERRA, MATÍAS; DAMIANO, ALICIA E; ANUD, CAROLINA; MEDINA, YOLLYSETH; SZPILBARG, NATALIA

BOGOTA

Simposio; III Simposio Colombiano de Placenta e Interacción Materno-Fetal; 2020

SOCIEDAD DE PLACENTA E INTERACCION MATERNO FETAL

Placental aquaporin 9 (AQP9) does not participate directly in the transfer of water between mother and fetus, its role in the human placenta is still unknown1. In other tissues, its participation in lactate transport as an alternative energy source to promote cell survival has been reported2,3. In this sense, our objective was to evaluate the participation of placental AQP9 in lactate transfer. Explants from normal term placentas were cultured in low glucose conditions with or without L-lactate, and in presence and absence of AQP9 inhibitor (0.5mM Phloretin). Cell viability and apoptotic parameters were evaluated (MTT and LDH assay, Bax / Bcl-2 expression and apoptotic nuclei). MTT levels decreased in low glucose cultured explants and apoptotic parameters increased compared to controls (n=5, p