Effect of alkaline gradient on clear renal cell carcinoma proliferation: role of isoform 1 of Na+/H+ exchanger function

DI GIUSTO G; CAPURRO C; MECHALI A; BELTRAMONE N; RIVAROLA V; CABRAL B; FORD P

CABA

Congreso; Reunion de Sociedades de Biociencias SAIC SAFIS SAI 2020; 2020

SAIC SAFIS SAI

The association between proliferation and intracellular pH elicits the possibility that extracellular pH (pHe) may modify cell survival. Moreover, as tumor extracellular acidity is a hallmark of cancer, is probable that pHe affects differently cancer or normal cells. Our previous studies showed that cells derived from renal cell carcinoma (RCC) were more sensible to cell death after 72h exposition to 9.6 mM NaOH (mild alkalosis) than normal cells. The aim of this study was to investigate if this alkaline condition also affects cell proliferation. We use three renal cell models: HK2, derived from normal human proximal epithelial cells, 786-O and Caki-1, both derived from human RCC. We exposed cells to media with 9.6 mM NaOH for 72h. Then, we estimated cell proliferation by BrdU experiments. Our results show at pH 7.4 both RCC derived cell lines proliferate more than normal HK2 cells (% BrdU+ cells: HK2= 31±2; 786-O= 46±3 and Caki-1= 58±3, p