Role of the caveolae in the formation of the placental vasculature

DAMIANO, ALICIA E.; REPPETTI, JULIETA; MARTINEZ, NORA

Bogotá

Simposio; III Simposio Colombiano de Placenta e Interacción Materno-Fetal; 2020

Sociedad Colombiana de Placenta e Interacción Materno-Fetal

Placental angiogenesis requires proliferation, migration, and differentiation of endothelial cells [1]. This process involves the formation of the placental microvasculature and macrovasculature and is a physiological process that must be finely regulated. There are large numbers of caveolae in the plasma membrane of endothelial cells [2]. Caveolae are lipid domains that have Caveolin-1 (Cav-1) as their marker protein. Cav-1 can bind different proteins and thus promote interactions between receptors, channels, and signaling proteins [3]. Our main objective was to evaluate the role of the caveolae in the formation of the placental vasculature. Placental microvascular endothelial cells (hPMEC) and the EA.hy926 cell line (ATCC®CRL-2922?) were used. Cav-1 gene and protein expression, and localization were evaluated. Cells were treated with 5mM methyl-β-cyclodextrin (MβCD) to disrupt caveolae [4]. Cell viability was assessed by MTT and cell migration by wound healing assay. Cav-1 was located in the plasma membrane of placental endothelial cells. MβCD significantly reduced cell migration in EA.hy926 cells (n=4; p