IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of estrogens on renal proximal tubule epithelial cells.
Autor/es:
SÁNCHEZ DAIANA S; IBARRA FERNANDO R; JOVE PILAR; AZURMENDI PABLO J; SILBERSTEIN CLAUDIA; SANDRA G VLACHOVSKY; ODDO ELISABET M
Reunión:
Congreso; Reunión de Sociedades de Biociencias 2020: SAIC, SAFIS, SAI; 2020
Resumen:
We have previously demonstrated that 17β-Estradiol (17βE) stimulates cell proliferation through classic estrogen receptors (ER) and the G protein-coupled estrogen receptor 1 (GPER-1), in primary cultures of human renal cortical tubular epithelial cells (HRTEC). We also observed that 17βE decreases the expression of Na+ K+ ATPase (NKA) in primary cultures. The aim of the present work is to study the effects of 17βE on cell proliferation and the expression of NKA in a human renal proximal tubular epithelial cell line (HK-2) and its comparison with previous results in HRTEC and in studies in vivo with ovariectomized (oVx) Wistar rats. HK-2 were treated with 17βE (10 nM, 24 h) with or without an agonist (G-1) or an antagonist (G-15) of GPER-1. Cell proliferation was measured by bromodeoxyuridine (BrdU) uptake. The expression of NKA was assayed by western blot. In HK-2, 17βE stimulated the BrdU uptake (26%) compared with control cells (p