Blood pressure reducing effect on the expression of proteins linked to sodium transport in peripheral blood lymphocytes in a rat model of salt-sensitive hypertension.

LUIS A DI CIANO; GOETTE NORA P; IBARRA FERNANDO R; AZURMENDI PABLO J; ARRIZURIETA ELVIRA E; SANDRA G VLACHOVSKY; ELISABET M ODDO; SILBERSTEIN CLAUDIA

Mar del Plata

Congreso; Reunion anual de sociedades de Biociencia; 2019

Sociedad Argentina de Investigación Clínica

Recent investigations have highlighted the role of the adaptive immune system in the genesis of high blood pressure. In previous work we described a model of salt-sensitive hypertension in ovariectomizedWistar rats in which we studied the expression of proteins related to sodium transport in peripheral blood lymphocytes (PBL). We showed that proteins such as Na+, K+-ATPase (NKA), cotransporter Na+, K+, 2Cl- (NKCC1), dopamine receptor 1(D1DR) and CD4+ T-cell surface protein responded differently to a high sodium intake (HS)in intact female (IF) or ovariectomized (oVx) rats. Aim: to determine whether the changes in the protein expression in PBL occur in response to the increase in blood pressure or secondary to the HS intake. For this purpose, IF and oVx rats, with normal sodium (NS) or HS intake, were treated with hydralazine (HZ), a peripheral vasodilator.Methods: Half of rats were ovariectomized at 60 days of life and at 145 days the IF and oVx were divided into two subgroups with either NS (0.24% NaCl) or HS (1% NaClin drinking water). At day 150 systolic bloodpressure (SBP, tail-cuff method) was recorded and blood samples were taken for PBLseparation (ficoll). Then, rats startedwith HZ treatment in drinking water (20 mg/kg/day). At day 157, SBP records and PBL samples were repeated. Protein expression was analyzed by Western Blot.Results: SBP (mmHg), IF NS: 131 ± 4.5 vs IF NS HZ: 117 ± 3.62, IF HS: 125 ± 3.87 vs IF HS HZ: 117 ± 6.77, oVx NS: 128 ± 4.03 vsoVx NS HZ: 113 ± 3.92, oVx HS: 148 ± 4.81*vsoVx HS HZ: 112 ± 3.82. * p